Device and method for the regeneration of biosensors

摘要

A device for the regeneration of a biosensor having an immobilized and biologically active material applied to the surface of a carrier that is suitable for interacting with a substance to be analyzed. The device comprises at least one injector through which, by means of a pump, at least one solution can be applied to the surface of the carrier for the purposes of rinsing the biologically active material.

主权项

1. A fluorescent excitation biosensor having a light source that is positioned on the backside of a surface, comprising an immobilized and biologically active material that is applied to the surface of a carrier and is suited to interact with a substance to be analyzed, wherein the surface of the carrier is an optically transparent semiconductor in form of a gallium nitride-, a zinc oxide- or a diamond coating, or a composition including these materials, epitaxially grown on a sapphire substance; the biosensor further including a regenerating device comprising:
at least two solution containers each containing different solutions, wherein at least one solution is a molecular-removal solution and at least one solution is a biologically active material; and an automated microfluid system integrated into the biosensor comprising: at least one injector through which, via a pump, at least one of the solutions is applied onto the surface of the carrier for rinsing the biologically active material; further comprising a valve arrangement, though which the solutions are applied successively by the injector and the pump to the surface of the carrier; and wherein the reagents and/or the carrier are temperature-controlled; and/or wherein a chamber with the carrier can be subjected to ultrasound.