método para teste de ácidos nucléicos em um suporte, kit para teste de ácidos nucléicos em um suporte, uso de um oligonucleotídeo marcado complementar a um trecho de nucleotídeos de um tipo único de base para teste da condição de ácidos nucléicos imobilizados em um suporte sólido através de ligação cruzada por calor ou luz ou através de imobilização química e método para análise de ácidos nucléicos

摘要

The present invention relates to a method for testing nucleic acids on a support, comprising the immobilization of one or more nucleic acids via crosslinking, wherein each of the immobilized nucleic acids includes a stretch of nucleotides of only one basetype, the provision of labeled oligonucleotides complementary to said stretch of nucleotides, and the determination of a value indicative for the condition of said nucleic acids. The present invention further relates to a kit for testing nucleic acids on a support comprising an array of nucleic acids immobilized on a solid support, wherein each of the immobilized nucleic acids includes a stretch of nucleotides of only one basetype and a labeled oligonucleotide complementary to said stretch of nucleotides. The invention additionally concerns the use of a labeled oligonucleotide complementary to a stretch of nucleotides of only one basetype for testing the condition of nucleic acids immobilized on a solid support.