Identification of isoprene synthase variants with improved properties for the production of isoprene

摘要

The invention provides for compositions and methods for producing isoprene using isoprene synthase variants with improved properties.

主权项

1. A recombinant host cell comprising a nucleic acid encoding a polypeptide having isoprene synthase activity, wherein the polypeptide comprises one or more amino acid substitution(s) at one or more residue position(s) corresponding to positions in the amino acid sequence of SEQ ID NO:1, wherein said one or more amino acid substitution(s) is selected from the group consisting of: X118E, X36W, X22K, X228Y, X448L, X488F, X467H, X331P, X448I, X392Y, X448V, X282H, X383Y, X511 Y, X448E, X376M, X488L, X461A, X414I, X282W, X493E, X392S, X448Q, X282Y, X537N, X447Y, X240C, X538R, and X510C, and wherein the polypeptide has increased kcat compared to a parent polypeptide which does not comprise said one or more amino acid substitution(s), and wherein said polypeptide has at least 80% sequence identity with SEQ ID NO:1.