| 摘要 |
FIELD: biotechnologies.SUBSTANCE: method of obtaining of biomass of activated autochthonic microorganisms-biodestructors of N-phosphon methyl glycine (glyphosate) is offered. Before cultivation the composition of the liquid medium is added by 0.05-0.4 mcg·cmof glyphosate and perfluordecalin emulsion stabilized by the emulsifier monostearate sorbitan. The final content of perfluordecalin in the liquid nutrient medium is 1.0% by weight, a monostearate sorbitan - 0.0005% by weight. Throughout all the cycle of cultivation fractionally, with the interval 2-4 hours, glyphosate is added with the increment 1.25-2.5 after each addition, starting from the amounts of glyphosate equal to its initial contents in the cultural medium.EFFECT: possibility to obtain in one cycle of deep cultivation the cultures of bacteria and micromycetes with the glyphosate resistance increased 150-200 times, and ability to destruction of glyphosate, 7-11 times exceeding the biodestructive activity of initial autochthonic isolates.3 tbl, 2 ex |
