RNA APTAMER ISOLATION VIA DUAL-CYCLE (RAPID) SELECTION

摘要

The present invention relates to a method for selecting an aptamer for a target molecule. The method involves providing a random oligonucleotide library comprising a plurality of unique random sequence oligonucleotides; providing a target mixture comprising at least one target molecule; and subjecting the random oligonucleotide library and the target mixture to at least one round of an aptamer isolation protocol to yield at least one aptamer for the target molecule, wherein a round of the aptamer isolation protocol comprises at least one selection cycle followed by an amplification cycle. The present invention also relates to systems and devices for implementing or performing the method of the present invention. The present invention further relates to using the method to isolate aptamers for high-throughput sequencing analysis and other aptamer analysis protocols.

主权项

1. A method for selecting an aptamer for a target molecule, said method comprising:
providing a random oligonucleotide library comprising a plurality of unique random sequence oligonucleotides; providing a target mixture comprising at least one target molecule; and subjecting the random oligonucleotide library and the target mixture to at least one round of an aptamer isolation protocol to yield at least one aptamer for the target molecule, wherein a round of the aptamer isolation protocol comprises at least one selection cycle followed by an amplification cycle, wherein said at least one selection cycle comprises: (i) contacting the random oligonucleotide library with the target mixture to bind oligonucleotides to the target molecule; and (ii) isolating the bound oligonucleotides to yield an enriched oligonucleotide pool comprising a plurality of high affinity oligonucleotides that bind with specificity to the target molecule; and wherein said amplification cycle comprises subjecting the enriched oligonucleotide pool to an amplification process to yield an amplified oligonucleotide pool comprising an increased number of copies of the plurality of high affinity oligonucleotides.