Reassortant influenza viruses for vaccines

摘要

The invention provides compositions and methods useful to prepare segmented, negative strand RNA viruses, e.g., orthomyxoviruses such as influenza A viruses, entirely from cloned cDNAs and in the absence of helper virus.

主权项

1. A method for producing reassortant influenza virus particles for preparation of a vaccine, comprising:
i) introducing a first set of expression vectors into cells containing a nucleoprotein and an RNA dependent RNA polymerase, wherein said vectors comprise genomic influenza vRNA segments or antigenomic cRNA segments of said genomic influenza vRNA segments; ii) culturing said cells in the absence of helper virus such that ribonucleoprotein complexes containing the genomic influenza vRNA segments are formed and reassortant influenza viral particles comprising the genomic influenza vRNA segments is assembled in said cells; and iii) recovering said reassortant influenza viral particles from said cells.