主权项 |
1. A method of making an electrochemical sensor strip, the method comprising:
depositing a first electrode on a base; depositing a second electrode on the base; applying a first reagent layer onto the first electrode, the first reagent layer comprising an electroactive organic molecule and an oxidoreductase configured to facilitate a redox reaction of an analyte; and applying a second reagent layer onto the second electrode, the second reagent layer consisting essentially of a redox pair comprising a first soluble redox species and a second soluble redox species, the redox pair being selected from a group consisting of an organotransition metal complex, a transition metal coordination complex, and mixtures thereof, wherein the first soluble redox species is configured to undergo a redox reaction opposite that of the analyte, and wherein the molar ratio of the first soluble redox species to the second soluble redox species is greater than about 1.2:1 prior to use of the sensor strip in an analysis. |