Wheat with increased resistant starch levels

摘要

A series of independent human-induced non-transgenic mutations found at one or more of the SBEII genes of wheat; wheat plants having these mutations in one or more of their SBEII genes; and a method of creating and finding similar and/or additional mutations of SBEII by screening pooled and/or individual wheat plants. The seeds and flour from the wheat plants of the present invention exhibit an increase in amylose and resistant starch without having the inclusion of foreign nucleic acids in their genomes. Additionally, the wheat plants of the present invention exhibit altered SBEII activity without having the inclusion of foreign nucleic acids in their genomes.

主权项

1. A wheat plant comprising a null mutation in each allele of an SBEIIa gene of the A, B, and D genomes, wherein the SBEIIa gene of the A genome encodes an SBEIIa polypeptide comprising a tryptophan to a stop mutation at an amino acid position corresponding to amino acid position 436 of SEQ ID NO: 2, wherein said wheat plant produces seed that germinates, and further wherein grain from said wheat plant has an increased amylose level as compared to grain from a wild type wheat plant.