AFLATOXIN NANOBODY IMMUNOABSORBENT AND IMMUNOAFFINITY COLUMN AND PREPARATION METHOD AND USE THEREOF

摘要

An aflatoxin nanobody immunoabsorbent and immunoaffinity column and preparation method and use thereof; the immunoabsorbent comprises a solid phase carrier and aflatoxin B1 nanobody 2014AFB-G15 coupled with the solid phase carrier, the 50% inhibiting concentration IC 50 of aflatoxin B1 nanobody 2014AFB-G15 to aflatoxin B1 being 0.66 ng/mL, and the cross-reactivity of aflatoxin B1 nanobody 2014AFB-G15 to aflatoxin B2, G1, G2, and M1 being 22.6%, 10.95%, 32.1% and 26% respectively; the amino acid sequence thereof is as represented by SEQ ID NO: 7, and the coding gene sequence thereof is as represented by SEQ ID NO: 8. The aflatoxin nanobody immunoaffinity column can be used for purification and concentration of sample extract prior to computer testing, and the immunoaffinity column can be reused repeatedly.