HIGH XYLANASE YIELD ASPERGILLUS NIGER AND APPLICATION THEREOF

摘要

Provided is a high xylanase yield Aspergillus niger strain SM24/a, accession number CGMCC No. 8671. The Aspergillus niger strain SM24/a can produce xylanase having a high tolerance to ethanol, acetic acid, furfural, vanillin, ferulic acid, and mixtures thereof, the enzyme activity of the resulting xylanase being very high, having solid state fermentation activity of up to 10801 IU/g, the optimum reaction pH value is 5.6, the optimum reaction temperature is 37ºC, and at 50ºC the residual rate of enzyme activity is 79.01%. In addition, 5-hydroxymethyl furfural has an inhibiting effect on the enzyme. Whether individually or in a mixture, the strength of 5-hydroxymethyl furfural shows a definite linear relationship with the rate of inhibition. Thus, also provided is an application of the xylanase as a qualitative and quantitative indicator of 5-hydroxymethyl furfural strength.