REAL-TIME DETECTION APPARATUS OF NUCLEIC ACID AMPLIFICATION PRODUCT

摘要

PROBLEM TO BE SOLVED: To provide a real-time detection apparatus of a nucleic acid amplification product capable of effectively excluding or reducing error factors on the apparatus without using a second fluorescent signal used for correction. SOLUTION: In the detection apparatus, fluorescence intensity from the nucleic acid amplification product in each wale 7A is detected in real time by each giving a temperature cycle to a plurality of wales 7A. A fluorescence measured value [DNA]raw obtained from the wale 7A and a fluorescence measured value [DNA]bg obtained from a connection wall of the periphery in the vicinity of the wale 7A are detected and the fluorescence measured value [DNA]bg is subtracted from the fluorescence measured value [DNA]raw to determine a fluorescence intensity [DNA]real of the wale 7A. COPYRIGHT: (C)2007,JPO&INPIT