发明名称 |
Method of production of recombinant proteins in eukaryote cells |
摘要 |
The invention provides a method for production of polypeptides in eukaryote microcarrier cell culture, the method comprising the steps of (i) Culturing cells expressing said polypeptide on microcarriers under conditions and at a setpoint temperature appropriate for expression of said polypeptide; (ii) Cooling the culture to a predetermined temperature below said setpoint; (iii) Sedimenting the microcarriers; and (iv) Harvesting all or part of the culture medium.
|
申请公布号 |
US2006194289(A1) |
申请公布日期 |
2006.08.31 |
申请号 |
US20060429558 |
申请日期 |
2006.05.05 |
申请人 |
NOVO NORDISK HEALTHCARE A/G |
发明人 |
KNUDSEN IDA M. |
分类号 |
C12P21/06;C07H21/04;C12N5/06;C12N5/08 |
主分类号 |
C12P21/06 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|