INDICATOR CELL LINES AND METHODS FOR MAKING SAME

摘要

Methods of making an indicator cell are described. The methods include, e.g., contacting a vertebrate cell comprising a functional endogenous target gene under control of an endogenous inducible promoter with a parvoviral vector comprising a construct comprising a targeting DNA sequence linked to a DNA encoding a reporter gene, wherein the construct enters the cell and undergoes homologous recombination with the target gene, thereby operably linking the reporter gene and the target gene; inducing expression of the target gene thereby causing expression of the reporter gene; and selecting the cell based on expression of the reporter gene.