IDENTIFICATION OF A TRANSFORMING FRAGMENT OF HERPES SIMPLEX TYPE 2 AND DETECTION THEREOF IN CLINICAL SPECIMENS

摘要

The present invention relates to oligonucleotide probes derived from HSV-2, capable of selectively hybridizing thereto and to a subsequence of HSV-2 Bgl II N from which the oligonucleotide probes were derived. Further, the invention relates to an optimized assay of nucleic acid amplification permitting the sensitive and specific detectio n in clinical speciments of HSV-2 as well as a specific typing of the HSV in the sample. The present invention further relates to kits for the detect-on and typing of the HSV in a sample. In addition, the invention provides the nucleic acid and amino acid sequence of a subsequence of HSV-2 Bgl II N having transforming activity. Further, the invention teaches diagnostic and therapeutic methods for genital cancer comprising the use of these sequences or ligands directed thereto.