发明名称 |
METHODS FOR DETERMINATION OF PROTEIN PHOSPHATASE ACTIVITY, AND USES IN PREDICTING THERAPEUTIC OUTCOMES |
摘要 |
One aspect of the present disclosure encompasses methods for determining a protein kinase or phosphatase activity in a biological sample, comprising: contacting in a reaction mix a first test sample and a fluorescently-labeled peptide substrate capable of being modified by a protein phosphatase or a protein kinase, contacting the reaction mix with a TiO2 matrix, thereby partitioning fluorescently-labeled phosphorylated peptide from fluorescently-labeled dephosphorylated peptide; and determining the fluorescence of the fluorescently-labeled dephosphorylated peptide, thereby determining a protein kinase or phosphatase activity. |
申请公布号 |
EP2183385(A4) |
申请公布日期 |
2010.11.10 |
申请号 |
EP20080796933 |
申请日期 |
2008.07.31 |
申请人 |
EMORY UNIVERSITY |
发明人 |
GOOCH, JENNIFER, L.;ROBERTS, BRIAN, R.;POHL, JAN |
分类号 |
C12Q1/48;C12N9/12;C12Q1/70 |
主分类号 |
C12Q1/48 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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