发明名称 |
MODULATION OF CELL GROWTH AND GLYCOSYLATION IN RECOMBINANT GLYCOPROTEIN PRODUCTION |
摘要 |
The present invention relates to methods for selecting between conditions which enhance cell growth or biomass generation and conditions which affect the N-glycosylation maturity of expressed glycoprotein produced by eukaryotic cells under fermentation culture conditions by adjusting the concentrations of iron, copper, zinc and manganses in the medium. Thus, in the methods of the present invention, the glycoprotein producing cells are cultured in a medium which is tailored to a desired end result. The invention also embraces the medium and the use thereof. |
申请公布号 |
WO2015128314(A1) |
申请公布日期 |
2015.09.03 |
申请号 |
WO2015EP53804 |
申请日期 |
2015.02.24 |
申请人 |
F. HOFFMANN-LA ROCHE AG;HOFFMANN-LA ROCHE INC.;LUDWIGS-MAXIMILIANS-UNIVERSITY MUNICH |
发明人 |
POPP, OLIVER;BEAUCAMP, NICOLA;DRABNER, GEORG;ESSLINGER, STEPHANIE |
分类号 |
C12P21/00 |
主分类号 |
C12P21/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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