METHODS FOR PROVIDING CYTOPLASMIC MALE STERILE PETROSELINUM CRISPUM PLANTS, CYTOPLASMIC MALE STERILE PETROSELINUM CRISPUM PLANTS AND SEEDS AND PLANT PARTS THEREOF

摘要

The present invention relates to methods for providing cytoplasmic male sterile Petroselinum plants, cytoplasmic male sterile Petroselinum crispum plants and to seeds, cells, tissues and plant parts thereof. Specifically the present invention relates to a method for providing a cytoplasmic male sterile Petroselinum crispum plant comprising: (a) providing first protoplasts obtained from a plant selected from the group consisting of Daucus carota L., Foeniculum vulgare Mill., Apium graveolens L., and Pastinaca sativa L., wherein the first protoplasts have a substantially inactivated nuclear genome and a substantially non-modified cytoplasm; (b) providing second protoplasts obtained from Petroselinum crispum wherein the second protoplasts have a substantially inactivated cytoplasm and a substantially non-modified nuclear genome; (c) fusing said first and second protoplasts; and (d) obtaining from the fusion product of said first and second protoplasts a cytoplasmic male sterile Petroselinum crispum plant.