Method of weak partitioning chromatography

摘要

This invention relates to methods of using weak partitioning chromatography for the purification of a product from a load fluid containing one or more impurities. Further, the invention relates to methods of weak partitioning chromatography defined by operating conditions which cause a medium to bind least 1 mg of product per mL of medium, or alternatively, defined by a partition coefficient of at least 0.1.

主权项

1. A method of recovering a purified product from a load fluid including one or more impurities, comprising the steps of:
passing the load fluid through a medium at operating conditions which cause the medium to bind at least 1 mg of product per mL of medium; and recovering the purified product in the column effluent during the load cycle and any essentially isocratic wash.