Methods of proliferating stem cells

摘要

The invention relates to methods of proliferating stem cells. More particularly, the invention relates to the use of glycosaminoglycans or proteoglycans to promote the growth of stem cells in ex vivo culture, while preserving their multipotentiality.

主权项

1. An in vitro culture of mesenchymal stem cells, the cell culture comprising:
(i) an isolated CD34+ fraction of multipotent human mesenchymal stem cells (hMSCs) expressing STRO-1 and having an ability to generate cartilage, bone, muscle, tendon, ligament and fat, and (ii) a cell culture medium comprising isolated heparin sulfate 2 (HS2) at a concentration that ranges between about 6.4 ng/ml to 20 μg/ml culture media, wherein hMSCs cultured in contact with HS2 for a given period of time undergo more population doubling than hMSCs without HS2.