COMPOSITION AND METHOD FOR GEL ELECTROPHORESIS WITH IN-SITU CALIBRATION

摘要

The invention relates to, among other things, a method for performing electrophoresis with in-situ calibration. The method includes combining a volume of a test sample with a volume or quantity of a calibrating sample to form a final volume, where the volume of the calibrating sample includes a known concentration of a calibrator and the final volume includes a known ratio of test sample to calibrating sample. The method also includes depositing a loading fraction in a receiving well of an electrophoretic gel, in which the loading fraction is a fraction of the final volume, and separating the loading fraction along a common separation lane of the electrophoretic gel such that components of the test sample and the calibrator are separated from one another along the common separation lane. The method also includes detecting the calibrator and separated components of the test sample within the common separation lane and measuring the level of the calibrator and separated components of the test sample based on the detecting, thereby performing electrophoresis with in-situ calibration.

主权项

1. A method for performing electrophoresis with in-situ calibration, the method comprising:
combining a volume of a test sample with a volume of a calibrating sample to form a final volume, wherein the volume of the calibrating sample comprises a known concentration of a calibrator and the final volume comprises a known volumetric ratio of test sample to calibrating sample; depositing a loading fraction in a receiving well of an electrophoretic gel, wherein the loading fraction is a fraction of said final volume; separating the loading fraction along a common separation lane of the electrophoretic gel such that components of the test sample and the calibrator are separated from one another along the common separation lane, wherein the one or more components of the test sample comprise lipoprotein particles and wherein the lipoprotein particles have not been subjected to separation by ultracentrifugation prior to said separating of the loading fraction; detecting the calibrator and separated components of test sample within the common separation lane; and measuring the level of the calibrator and separated components of the test sample based on said detecting, thereby performing electrophoresis with in-situ calibration.