摘要 |
<p>Carrying out homogeneous immunoassay format with electrochemical detection, in a solution, comprises combining two different conjugates as reagents with a sample or a sample-buffer mixture. The first conjugate comprises a redox marker and an analyte molecule. The second conjugate comprises an anti-redox marker antibody or its specific binding fragment, and a molecule binding specifically to the analyte. Independent claims are also included for: (1) a redox marker antibody or its fragment, which binds specifically to the redox marker, comprising ferrocene or its derivatives, osmium-bipyridine-complex or other osmium-based complexes, ruthenium-bipyridyl-complex or other ruthenium-based complexes, p-aminophenol, hexacyanoferrate (II/III), quinone or other redox markers suitable for electrochemical immunoassays, where the redox activity of the bound redox marker is largely suppressed (quenched), preferably upto greater than 90%, preferably greater than 95%, or completely; and (2) a bispecific antibody conjugate for carrying out the above method, comprising the redox marker-antibody or its fragment, and an anti-analyte-antibody or its fragment.</p> |