The invention discloses a cell culture process comprising culturing cells in the presence of betaine to obtain a glycoprotein with a glycoform composition comprising increased percentage of afucosylated glycans. Further, the invention 5 discloses a cell culture process comprising manganese, galactose and betaine for obtaining a glycoprotein with a glycoform composition comprising target values of mannosylated, galactosylated and afucosylated glycans.
