INTEGRATED DEVICE FOR REAL TIME QUANTITATIVE PCR

摘要

A method for real-time quantitative detection of single-type, target nucleic acid sequences amplified using a PCR in a microwell, comprising introducing in the microwell a sample comprising target nucleic acid sequences, magnetic primers, and labelling probes; performing an amplification cycle to form labelled amplicons; attracting the magnetic primers to a surface through a magnetic field to form a layer including labelled amplification products and free magnetic primers; and detecting the labelled amplification products in the layer with a surface-specific reading method.

主权项

1. A cell for performing real time quantitative PCR, comprising:
a microwell having a surface; a magnetic field source having a width and extending adjacent to the surface and configured to attract magnetic primers to the surface and generate in the microwell a multilayer of magnetic amplification products and a labelled probe hybridized to the multilayer, the multilayer having a thickness; and a spatially discriminating detection device having a focal plane and a vertical resolution comparable to the thickness of the multilayer.