Endotoxins having nematocidal activity and methods of use thereof

摘要

The invention provides novel δ-endotoxin polypeptides, and variants and fragments thereof, obtained from Bacillus thuringiensis having pesticidal activity against nematodes. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, biopesticide compositions, expression cassettes, and transformed microorganisms and plants comprising a nucleic acid of the invention. These compositions find use in methods for controlling pests, especially plant parasitic nematodes.

主权项

1. An expression cassette comprising a heterologous promoter operably linked to a polynucleotide comprising a nucleotide sequence selected from the group consisting of:
(a) a nucleotide sequence comprising SEQ ID NO: 2; (b) a nucleotide sequence encoding a polypeptide comprising the amino acid sequence of SEQ ID NO: 1; and (c) a nucleotide sequence encoding a polypeptide comprising residues 23-684 of SEQ ID NO: 1, wherein said amino acid sequence has nematicidal activity.