Library Compositions and Methods for Acyclic Identification of Aptamers

摘要

The present invention provides improved aptamer libraries useful for discovery of aptamers that have high binding affinity for a single or a plurality of targets. The libraries contain higher copies of each member candidate such that they are more likely to be available to the application of acyclic identification methods that obviate the most time-consuming and costly step in traditional SELEX method, the multiple cycles of evolutionary selection.

主权项

1. A method for identifying an aptamer that binds to a target comprising the steps of:
(a) providing a starting library of a starting pool of aptamer candidates; (b) reducing the sequence complexity of said starting library of aptamer candidates by a factor of at least about 100 to an intermediary pool of surviving aptamer candidates; (c) amplifying said intermediary library of surviving aptamer candidates to an over-represented library of said surviving aptamer candidates such that, for every 100 pmol of said surviving aptamer candidates, each surviving aptamer candidate has, on average, at least about three copies; (d) contacting said over-represented library with a target under a buffer condition that allows binding between members of said over-represented library and said target; (e) isolating at least one aptamer candidate that is bound to said target; and (f) determining the sequence of said bound aptamer candidate.