BISPECIFIC EGFR/IGFIR BINDING MOLECULES

摘要

The present invention relates to bispecific molecules comprising an EGFR binding domain and a distinct IGFIR binding domain for use in diagnostic, research and therapeutic applications. The invention further relates to cells comprising such proteins, polynucleotide encoding such proteins or fragments thereof, and vectors comprising the polynucleotides encoding the innovative proteins. Exemplary bispecific molecules include antibody-like protein dimers based on the tenth fibronectin type III domain.

主权项

1. A method for treating a hyperproliferative disorder in a subject comprising administering to a subject in need thereof a therapeutically effective amount of an antibody-like protein comprising a tenth fibronectin type III domain (10Fn3) that binds EGFR with a KD of less than 500 nM, wherein the EGFR binding 10Fn3 comprises a BC loop having the amino acid sequence XgWAPVDRYQXh (SEQ ID NO: 137), a DE loop having the amino acid sequence XiRDVYXj (SEQ ID NO: 138), and an FG loop having the amino acid sequence XkDYKPHADGPHTYHESXl (SEQ ID NO: 139); and
wherein X is any amino acid and a, b, c, d, e, f, g, h, i, j, k, and l are integers independently selected from 0 to 5.