| 摘要 |
Provided are: a protein complex capable of selectively and asymmetrically oxidizing one of enantiomers of a secondary alcohol without adding a coenzyme and having an asymmetric oxidation activity in a water-soluble solvent system in the presence of oxygen; a method for producing the same; and a production method relating to coating of the protein complex with a high molecular compound. A method for producing a protein complex capable of catalyzing an asymmetric oxidation reaction, said method being characterized by comprising: a first step that comprises a treatment for enclosing a crude water-soluble protein derived from an animal or plant in a gel, a treatment for air-oxidizing the gel, and a treatment for eluting the protein complex from the gel into an aqueous solution; and a second step that comprises a treatment for applying gravity to the aqueous solution of the protein complex to concentrate and precipitate the protein complex, a treatment for redissolving the protein complex precipitate in an aqueous glycine sodium hydroxide solution of about 0.5 mM and allowing the same to homogeneously coexist with a high molecular compound, and a treatment for re-precipitating the coexistence solution and dehydrating and drying the same to give the protein complex coated with the high molecular compound. |
