Particle quantifying systems and methods using acoustic radiation pressure

摘要

The present invention comprises methods and systems that use acoustic radiation pressure.

主权项

1. A method for quantifying the amount of analyte bound to a particle in a flow cytometer comprising:
binding a particle having a known amount of a calibration dye and the particle having a specificity for an analyte to the analyte having a lifetime related signal to form an analyte-particle complex; acoustically and hydrodynamically focusing the analyte-particle complex; passing the analyte-particle complex through an interrogation zone through which a light from a light source is passed wherein the interrogation zone is located within a flow cytometer; measuring the signal related to the binding event in the interrogation zone; measuring an overlapping signal from the calibration dye; and calculating the amount of analyte present by comparing the analyte related signal to the calibration dye signal.