| 摘要 |
The invention provides a non-naturally occurring microbial organism having a methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2-hydroxyisobutyrate pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in a methacrylic acid pathway. The invention additionally provides a method for producing methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2-hydroxyisobutyrate. The method can include culturing methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2-hydroxyisobutyrate producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a methacrylic acid pathway enzyme in a sufficient amount to produce methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2-hydroxyisobutyrate, under conditions and for a sufficient period of time to produce methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2-hydroxyisobutyrate. |
| 主权项 |
1. A non-naturally occurring bacterial organism having a methacrylic acid pathway, said bacterial organism comprising at least one exogenous nucleic acid encoding a methacrylic acid pathway enzyme expressed in a sufficient amount to produce methacrylic acid, said methacrylic acid pathway comprising a pathway selected from:
(a) citramalate synthase, citraconate-forming citramalate dehydratase, and a decarboxylase of a C1-C6 carboxylate; (b) citramalate synthase, citraconate-forming citramalate dehydratase, citraconate isomerase, and a decarboxylase of a C1-C6 carboxylate; (c) citramalate synthase, mesaconate-forming citramalate dehydratase, citraconate isomerase, and a decarboxylase of a C1-C6 carboxylate; (d) citramalate synthase, mesaconate-forming citramalate dehydratase, and a decarboxylase of a C1-C6 carboxylate; (e) citramalyl-CoA lyase, citramalyl-CoA transferase, synthetase or hydrolase, citraconate-forming citramalate dehydratase and a decarboxylase of a C1-C6 carboxylate; (f) citramalyl-CoA lyase, citramalyl-CoA transferase, synthetase or hydrolase, citraconate-forming citramalate dehydratase citraconate isomerase, and a decarboxylase of a C1-C6 carboxylate; (g) citramalyl-CoA lyase, citramalyl-CoA transferase, synthetase or hydrolase, mesaconate-forming citramalate dehydratase, and a decarboxylase of a C1-C6 carboxylate; (h) citramalyl-CoA lyase, citramalyl-CoA transferase, synthetase or hydrolase, mesaconate-forming citramalate dehydratase, citraconate isomerase, and a decarboxylase of a C1-C6 carboxylate; (i) aconitate decarboxylase, itaconate isomerase, and a decarboxylase of a C1-C6 carboxylate; (j) aconitate decarboxylase, itaconate isomerase, citraconate isomerase, and a decarboxylase of a C1-C6 carboxylate; (k) aconitate decarboxylase, itaconyl-CoA transferase, synthetase or hydrolase, citramalyl-CoA dehydratase, citramalyl-CoA transferase, synthetase or hydrolase, citraconate-forming citramalate dehydratase, and a decarboxylase of a C1-C6 carboxylate; (l) aconitate decarboxylase, itaconyl-CoA transferase, synthetase or hydrolase, citramalyl-CoA dehydratase, citramalyl-CoA transferase, synthetase or hydrolase, citraconate-forming citramalate dehydratase, citraconate isomerase, and a decarboxylase of a C1-C6 carboxylate; (m) aconitate decarboxylase, itaconyl-CoA transferase, synthetase or hydrolase, citramalyl-CoA dehydratase, citramalyl-CoA transferase, synthetase or hydrolase, mesaconate-forming citramalate dehydratase, and a decarboxylase of a C1-C6 carboxylate; and (n) aconitate decarboxylase, itaconyl-CoA transferase, synthetase or hydrolase, citramalyl-CoA dehydratase, citramalyl-CoA transferase, synthetase or hydrolase, mesaconate-forming citramalate dehydratase, citraconate isomerase, and a decarboxylase of a C1-C6 carboxylate. |
