Massive parallel method for decoding DNA and RNA

摘要

This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogues which comprise unique labels attached to the nucleotide analogue through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3′-position of the deoxyribose.

主权项

1. A method of sequencing nucleic acid, comprising:
a) reacting a DNA strand containing an azido group with a phosphine; b) growing a double-stranded DNA containing said DNA strand by incorporating a fluorescently labeled nucleotide into said strand; and c) identifying the fluorescently labeled nucleotide, so as to sequence the nucleic acid.