| 摘要 |
Provided are a recombinant collagenase that has stable specific activity and an enzyme agent for cell separation, the enzyme agent including the recombinant collagenase. A recombinant collagenase that is derived from a Grimontia-hollisae-derived collagenase that includes, from the N-terminus to the C-terminus thereof, a collagenase catalytic domain, a linker region sequence, and a pre-peptidase C-terminal domain. The Grimontia-hollisae-derived recombinant collagenase is characterized by not including at least the pre-peptidase C-terminal domain. The recombinant collagenase that is obtained has high and stable specific activity. |
