CONTINUOUS BIOTRANSFORMATION OF SUBSTITUTED AROMATIC CARBOXYLIC ACIDS TO THEIR SELECTIVE ALDEHYDES AND ALCOHOLS

摘要

The present invention discloses a novel method for biological reduction of the carboxylic acids to their corresponding aldehydes and/or alcohols with high productivity and high yield by using fungus in the category of basidiomycetes. This reduction is specific and selective for its functional group (—COOH), without affecting other functional group such as—R groups (—OH, —NH2, -alkyl, -alkyoxy) and their position, number on aromatic ring. The method of the invention relates to reduction of aryl acids to aldehyde and/or alcohols by employing a white rot fungus—Pycnoporus cinnabarinus, an organism of basidiomycete species, grown in vessel/column. The biotransformation was performed in vessel/column/fermentor with pH control, dissolved oxygen, membrane system, product extractor is attached.

主权项

1. A method for biological reduction of the aryl carboxylic acids to their corresponding aldehydes and/or alcohols with high purity and yield comprising:
a) preparing the spore suspension of the culture Pycnoporus cinnabarinus (NCIM-1181); b) culturing the fungus Pycnoporus cinnabarinus (NCIM-1181) from the spore suspension in a fermentor containing culture medium of pH in the range of 2-6 for 48-96 hrs to obtain cell pellets for biotransformation; c) adding aryl carboxylic acid substrate or derivatives thereof, to the fermentor of step (b) to obtain the final concentration of 1-3 g/L of the aryl carboxylic acid substrate; wherein aryl carboxylic acid substrate is consumed for 50-90 hrs to obtain a fermentation broth containing an aldehyde and/or alcohol product; d) filtering the fermentation broth to obtain a permeate and a retentate fraction; e) passing the permeate fraction through capture column of the fermentor for selective extraction of the aldehyde and/or alcohol product obtained in step (c); f) eluting the capture column with an organic solvent to obtain aldehyde and/or alcohol product in high concentration; and g) crystallizing the aldehyde and/or alcohol product from step (f) to obtain pure aldehyde and/or alcohol product.