摘要 |
The invention provides a codon optimised tetracycline repressor (Tet R) sequence comprising the nucleic acid sequence of SEQ ID NO:4 or SEQ ID NO: 5, the sequence being optimised for expression in human cells. The sequence is preferably administered in a vector, which can be a plasmid vector. The vector may also include CMV promoter sequences and lentiviral genes. The invention also provides a cell comprising the vector and a method of transfecting a cell with the vector. |