BONE MORPHOGENIC PROTEIN BINDING PEPTIDE

摘要

A cyclized peptide designated BMP Binding Peptide (BBP) is a synthetic peptide that avidly binds rhBMP-2, as do endogenous forms of BBP, and sequence conservation between species results in a variety of useful BBP compositions. BBP increases the over-all osteogenic activity of rhBMP-2, increases the rate at which rhBMP-2 induces bone formation, and BBP induces calcification alone. Compositions and substrates including BBP, and methods of using BBP are useful in therapeutic, diagnostic and clinical applications requiring calcification and osteogenesis.

主权项

1. A method of obtaining pro-osteogenic mesenchymal stem cells for use in enhancing the rate or degree of osteogenesis or calcification in vertebrate tissue comprising:
(a) treating mesenchymal stem cells with one or more of TGF-beta, BMP-2, BMP-4, BMP-7 or demineralized bone matrix; (b) treating mesenchymal stems cells with a peptide having the amino acid sequence of SEQ ID No. 13, or a fragment thereof, wherein said peptide or fragment increases the degree or rate of osteogenesis or calcification.