发明名称 |
METHOD FOR PRODUCING 3D CELL CULTURE |
摘要 |
Provided is a novel method for producing a three-dimensional cell culture construct. A method for producing a three-dimensional cell culture construct including at least two or more laminated cell layers includes the following: seeding coated cells in which a cell surface is coated with a coating film containing an extracellular matrix component; culturing the seeded coated cells in a culture medium; and using at least a portion of the culture medium continuously for 5 days or more. A method for producing a three-dimensional cell culture construct including at least two or more laminated cell layers includes the following: seeding coated cells in which a cell surface is coated with a coating film containing an extracellular matrix component; and culturing the seeded coated cells in a culture medium. The culture process of the coated cells includes culturing the coated cells in 70 μL or more of the culture medium per 1×104 cells. |
申请公布号 |
US2015247118(A1) |
申请公布日期 |
2015.09.03 |
申请号 |
US201314427536 |
申请日期 |
2013.09.12 |
申请人 |
OSAKA UNIVERSITY ;MITSUBISHI PAPER MILLS LIMITED |
发明人 |
Akashi Mitsuru;Matsusaki Michiya;Matsuzawa Atsushi |
分类号 |
C12N5/00 |
主分类号 |
C12N5/00 |
代理机构 |
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代理人 |
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主权项 |
1. A method for producing a three-dimensional cell culture construct comprising at least two or more laminated cell layers,
the method comprising: seeding coated cells in which a cell surface is coated with a coating film containing an extracellular matrix component; culturing the seeded coated cells in a culture medium; and using at least a portion of the culture medium continuously for 5 days or more. |
地址 |
Osaka JP |