Selective gene expression in plants

摘要

This invention discloses methods for providing plants with selective expression of a target RNA and methods for evaluating an expression pattern of a target RNA in a plant. Also disclosed are transgenic plants having recombinant DNA for expressing a protein using a promoter functional in multiple tissues, and recombinant DNA for suppressing expression of the protein using a promoter functional in fewer than the multiple tissues.

主权项

1. A method of providing plants with selective expression of an exogenous RNA, comprising:
a) providing a set of first parent plants each expressing a recombinant DNA construct comprising a promoter operably linked to (i) DNA encoding an exogenous RNA, wherein said exogenous RNA is different for each of said first parent plants, and (ii) DNA encoding either a 5′ UTR or a 3′ UTR which is transcribed to a target RNA, wherein said target RNA is common to the set of first parent plants, and wherein said exogenous RNA and said target RNA are on the same RNA transcript; b) providing multiple second parent plants each expressing a recombinant DNA construct comprising a second promoter operably linked to DNA for suppressing expression of the target RNA, wherein each said second promoter is non-constitutive and is different for each of said multiple second parent plants; c) hybridizing said first parent plants and each of said multiple second parent plants to generate a heterogeneous population of hybrid progeny with regard to expression of said exogenous RNA; and d) producing a hybrid progeny with selective expression of said exogenous RNA relative to expression in said first parent plants.