能产生比小鼠ＩgＥ量高出许多的人源化ＩgＥ之基因转殖动物;TRANSGENIC ANIMALS CAPABLE OF PRODUCING HUMANIZED IGE AT MUCH HIGHER LEVELS THAN MOUSE IGE

摘要

本发明涉及建构非人类之基因转殖动物，在此动物的内源免疫球蛋白Cγ恒定区中的一个编码序列的基因组被人类的免疫球蛋白Cε恒定区的编码序列所取代。一优选的基因转殖动物是在其基因组中的Cγ1恒定区和Cκ恒定区分别被人的免疫球蛋白Cε恒定区和人的免疫球蛋白Cκ恒定区替换的小鼠。此基因转殖小鼠经过免疫后会产生分泌人源化IgE的B细胞和抗原特异性的人源化IgE。本发明还涉及应用这种基因转殖动物，藉由使用融合瘤和其他相关技术来制备含有人源化IgE的血清、含有抗原特异性的人源化IgE抗血清和抗原特异性的人源化IgE单株抗体。 This invention pertains to the construction of transgenic non-human animals, in whose genome the coding sequences of one of the animal’s endogenous immunoglobulin Cγ constant regions are replaced by human immunoglobulin Cε constant region coding sequences. One preferred transgenic animal is a mouse, in whose genome the Cγ1 constant regions are replaced by the human immunoglobulin Cε constant regions and the Cκ constant region is replaced by the human immunoglobulin Cκ