Method for producing glycolic acid using an inducible promoter

摘要

The present invention relates to use of inducible promoters in the production of glycolic acid by fermentation. The present invention concerns a method for the production of glycolic acid in a fermentative process comprising the following steps:
culturing a modified microorganism in an appropriate culture medium comprising a source of carbon,modulating in said microorganism the expression of a target gene with an external stimulus, andrecovering glycolic acid from the culture medium,

wherein in said modified microorganism, the expression of at least one gene involved in glycolic acid production is under the control of a heterologous inducible promoter whose activity is modulated with said external stimulus.
;The invention also concerned the modified microorganism used in the method of glycolic acid production.

主权项

1. A method for producing glycolic acid in a fermentative process comprising:
culturing a modified microorganism in an appropriate culture medium comprising a source of carbon, modulating in said microorganism an expression of a target icd gene with an external stimulus, and recovering glycolic acid from the culture medium,wherein, in said microorganism belonging to the Enterobacteriaceae genus, expression of the target icd gene involved in glycolic acid biosynthesis pathway is under control of a heterologous inducible promoter having activity that is modulated with said external stimulus, wherein said heterologous inducible promoter is selected from the group consisting of: a promoter regulated by a modified repressor of phage lambda, a modified lac promoter regulated by a temperature sensitive lac repressor, and a promoter having activity that is modulated with chemical external stimulus.