Enhanced zein reduction in transgenic corn seed

摘要

Anti-sense-oriented RNA gene suppression agents in the form of a loop of anti-sense-oriented RNA is produced in cells of transgenic organisms, e.g. plants, by transcription from a recombinant DNA construct that comprises in 5′ to 3′ order a promoter element operably linked to more than one anti-sense-oriented DNA element and one or more complementary DNA elements.

主权项

1. A method for generating maize seeds having at least one of enhanced lysine, enhanced oil and enhanced tryptophan comprising the steps of: a) transforming a maize plant cell with the recombinant DNA construct for suppression of a plurality of maize zein target genes, comprising in 5′ to 3′ order a promoter element operably linked to a plurality of anti-sense-oriented DNA elements from a plurality of maize zein target genes, the anti-sense-oriented DNA elements designated in 5′ to 3′ order element 1, element 2, and a plurality of sense-oriented DNA elements from a plurality of maize zein target genes, designed in 5′ to 3′ order element 3, element 4, wherein the anti-sense-oriented DNA element 2 has at least a portion of its sequence at its 3′ end that is not complementary either to itself or to the sense-oriented DNA element 3, and wherein sense-oriented RNA transcribed by the sense-oriented DNA elements is complementary to the 5′-most end of anti-sense-oriented RNA transcribed by the anti-sense-oriented DNA elements; and b) regenerating the maize plant cell into a fertile transgenic maize plant, wherein the maize plant contains the construct in its genome; and c) harvesting seed from the maize plant, wherein the seed has at least one of enhanced lysine, enhanced oil and enhanced tryptophan relative to seed of the same variety not transformed with the construct.