主权项 |
1. A pH-sensitive fluorescent dye compound of structural formula (I): wherein R1 is alkoxy or thioalkyl; R2 and R6, which may be the same or different, are each independently H, halogen, —ORa, —SRa, —NRaRb, or an electron donating group; R3 is —NR′R″, wherein R′ and R″, which may be the same or different, are each independently alkyl or substituted alkyl; R4 is selected from the group consisting of alkyl and substituted alkyl; R5 is selected from the group consisting of alkyl; substituted alkyl; alkenyl; substituted alkenyl; acyl; aryl; substituted aryl; carboxyalkyl; heteroaryl; substituted heteroaryl; heterocyclyl; substituted heterocyclyl; alkylcarboxy; alkylalkoxycarbonyl; alkylaminocarbonyl; alkylaryloxycarbonyl; alkylheteroaryl; (CH2),nCO(O)R; (CH2),nC(O)R; (CH2)nC(O)NHR; (CH2)nC(O)NRRc; wherein n is an integer from 1 to 6, and R and Rc, which may be the same or different, are each independently H, alkyl, substituted alkyl, aryl, substituted aryl, heteroaryl, substituted heteroaryl, substituted amino, alkylaminocarbonyl, aminodextran, amide, a protein, a lipophilic group, or Rd, wherein Rd is (CH2)nC(O)NHR, wherein n is an integer from 1 to 6, and Rx is a reactive group; -L-Rx; and -L-Sc, wherein L is a linker, Rx is a reactive group, and S, is a conjugated substance; Ra is H, alkyl, or substituted alkyl; and Rb is alkyl or substituted alkyl. |