发明名称 |
Development and use of cysteine-labeled fluorescent probes of unbound analytes |
摘要 |
A method for high throughput discovery of proteins fluorescently labeled at a cysteine residue and that undergo a change in fluorescence ratio at 2 wavelengths upon binding an unbound analyte is described. Probes are disclosed which are labeled at a cysteine residue and also probes labeled at both cysteine and lysine with two different fluorophores. These probes are useful for characterization and measurement of hydrophobic species in a fluid sample, particularly characterization and measurement of levels of unbound free fatty acids. A profile of unbound free fatty acids can be determined for an individual which can be used to determine the individual's relative risk for disease. |
申请公布号 |
EP2910932(A2) |
申请公布日期 |
2015.08.26 |
申请号 |
EP20140187900 |
申请日期 |
2009.01.16 |
申请人 |
FFA SCIENCES, LLC |
发明人 |
KLEINFELD, ALAN, MARC;HUBER, ANDREW, HENRY;KAMPF, JAMES, PATRICK;KWAN, THOMAS;ZHU, BAOLONG |
分类号 |
G01N21/76;C07K14/00;G01N33/68 |
主分类号 |
G01N21/76 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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