Method for production of lactoferrin

摘要

The invention concerns a method for production of lactoferrin comprising at least the steps of: a) disposing of raw material that have not been treated at a temperature greater than 500C, b) submitting this raw material to a treatment in order to obtain a solution of Lactenin (LN) or Milk Basic Protein (MBP), c) submitting this LN or MBP solution to a step of purification on a cation exchange resin equilibrated with an acetate buffer at a pH between 4 and 9 and eluted with different buffer solutions containing different solute concentrations, d) and collecting a fraction containing Lactoferrin having more than 95% of purity, having no polymers and substantially free of LPS, endotoxins and angiogenin. It also concerns the Lactoferrin obtained having more than 95% of purity, substantially free of LPS, endotoxins and angiogenin with an iron saturation level comprised between 9% to 15%.

主权项

1. A method for production of Lactoferrin comprising:
a) disposing of a raw material that has not been treated at a temperature greater than 50° C., b) extracting the raw material on a cation exchange resin with an excluded solute concentration solution in order to obtain a solution of Lactenin (LN) or Milk Basic Protein (MBP), wherein the excluded solute is sodium chloride, c) purifying the LN or MBP solution on a cation exchange resin equilibrated with an acetate buffer at a pH between 4 and 9 by eluting with different buffer solutions containing different solute concentrations, and d) collecting a fraction of the solution containing the Lactoferrin, the Lactoferrin having a purity of more than 95%, having no polymers, and being substantially free of LPS, endotoxins and angiogenin.