Sequences, antibodies, methods and kits for detection and in vitro assay of periostin, in order to provide a diagnosis, follow-up or prognosis of diseases and biological phenomena involving periostin

摘要

Detection sequences of periostin having from 6 to 30 amino acids and which include all or part of peptide sequence SEQ ID No. 1, SEQ ID No. 2 or SEQ ID No. 3, or a homologous peptide sequence. Antibodies suitable for specifically recognizing minimally one of these detection sequences, including an anti-periostin antibody, are disclosed. Periostin detection and dosage processes and kits that use these antibodies are also disclosed. A periostin marker for early diagnosis, tracking and prognosis of pathologies that involve periostin, including benign osteolysis such as inflammatory diseases, including osteoarticular diseases, and malignant osteolysis, such as cancers with bone metastases, is provided.

主权项

1. An isolated anti-periostin antibody, or one of its functional fragments, said antibody or its functional fragments able to recognize only an epitope comprised in the peptide consisting of SEQ ID No. 1, SEQ ID No. 2, or SEQ ID No. 3, wherein said functional fragments are selected from the group consisting of fragment Fv, fragment Fab that contains a site of recognition specific to the antigen, and fragment F(ab′)2 that contains at least two recognition sites specific to the antigen.