Células deficientes em fucosilação

摘要

A cell as provided that expresses a GDP-4-keto-6-deoxy-mannose-3,5-epimerase-4-reductase (FX) protein with a modification, wherein the cell lacks or substantially lacks the ability to fucosylate a glycoprotein at a first temperature, but does not lack or does not substantially lack the ability to fucosylate the glycoprotein at a second temperature. Methods for making antigen binding proteins are also provided which use the cell.