MICROFLUIDIC CHIP FOR MULTI-ANALYTE DETECTION

摘要

A microfluidic chip and compatible bio-sensor are provided to detect and/or quantify an analyte in a sample fluid, and preferably to simultaneously quantify multiple analyte(s) in a sample fluid volume. A fluid volume containing microbeads is flowed through an elongate planar sample chamber of the microfluidic chip. Microbead traps or barriers in the sample chamber retain the microbeads. The sample fluid volume is Excitation flowed through the sample chamber. A conjugate specific to the analyte is and labeled with a fluorophore is introduced into the sample chamber. In the biosensor, an excitation wavelength is directed parallel to the plane of the sample chamber. Any fluorescent emissions from the sample chamber are detected in a direction substantially orthogonal to the plane of the sample chamber by a photodetector, and correlated to an amount or concentration length of sample chamber of analyte in the sample fluid volume.

主权项

1. A microfluidic chip for use with an assay using a plurality of microbeads, the microfluidic chip comprising:
(a) two opposed planar sides defining therebetween an elongate planar sample chamber in fluid communication with an inlet and an outlet, wherein the direction from the inlet to the outlet defines a downstream longitudinal direction and a lateral direction perpendicular to the longitudinal direction in the plane of the sample chamber; and (b) at least one microbead trap within the sample chamber between the inlet and the outlet.