METHOD FOR SPECIFICALLY DETECTING A MATRIX METALLOPROTEINASE (MMP) WHICH IS ONLY OF INTEREST IN THE ACTIVE FORM THEREOF, IN A SAMPLE

摘要

The present invention relates to a method that allows to detect the active form of a specific matrix metalloproteinase and its use in diagnostic methods.

主权项

1. An in vitro method for specifically detecting in a biological sample a matrix metalloproteinase (MMP) of interest only in its active form, comprising
a) a step of placing the biological sample in contact with a ligand of the MMP of interest capable of binding to the free active site of the MMP; b) subsequently to or simultaneously with step a), a step of placing the result of step a) in contact with an antibody specific for the MMP of interest; and c) a step of detecting the ternary complex between the MMP of interest, the ligand of the MMP of interest, and the antibody specific for the MMP of interest,and wherein the ligand comprises a phosphinic pseudopeptide inhibitor.