METHOD FOR PROTEIN ANALYSIS

摘要

The present invention relates to a method for protein analysis in which the proteins to be analyzed are displayed on a population of discrete and dispersible structures, such as beads or other particles, for subsequent affinity reactions and analysis/detection. More closely the invention relates to a method for protein analysis comprising providing a denatured protein sample in the presence of a denaturing agent, the method comprising the following steps; a) contacting said protein sample with a population of discrete and dispersible protein-binding structures to capture a plurality of different proteins on said structures, b) removal of denaturing agent to display protein-and protein-feature specific epitopes from said sample on said structures; c) performing affinity probing targeted to a sub-group of said protein or protein feature specific epitopes requiring two or more specificity building events per targeted protein or protein feature generating a target specific nucleic acid molecule or sequence for each of the targeted proteins or protein-features; d) liberating said protein-or protein-feature specific nucleic acid sequences or their complements from said structures; e) optionally amplifying said nucleic acid sequences; f) analysing the generated nucleic acid molecules by a quantitative and sequence specific nucleic acid detection method; and g) determining therefrom the quantity or presence of targeted proteins or protein features in said sample.