| 发明名称 | MGMT-based method for obtaining high yields of recombinant protein expression | ||
| 摘要 | The present invention relates to a novel enhancer of protein production in host cells. It discloses a vector for expressing recombinant proteins in these cells, comprising a nucleotide sequence encoding a) a secretion peptidic signal, b) a 6-methylguanine-DNA-methyltransferase enzyme (MGMT, EC 2.1.1.63), a mutant or a catalytic domain thereof, and c) a recombinant protein. Said MGMT enzyme is preferably the so-called SNAP protein. | ||
| 申请公布号 | US9109219(B2) | 申请公布日期 | 2015.08.18 |
| 申请号 | US201113824476 | 申请日期 | 2011.12.09 |
| 申请人 | INSTITUT PASTEUR | 发明人 | Despres Philippe;Paulous Sylvie;Crublet Elodie |
| 分类号 | C12N9/96;C12N1/20;C12N1/00;C12N15/00;C12N5/07;C12N9/10;C12N15/62;C12N15/85 | 主分类号 | C12N9/96 |
| 代理机构 | Law Office of Salvatore Arrigo and Scott Lee, LLP | 代理人 | Law Office of Salvatore Arrigo and Scott Lee, LLP |
| 主权项 | 1. A vector for expressing recombinant proteins in isolated host cells, comprising a nucleotide sequence encoding in a single open reading frame, from 5′ to 3′: a) a peptidic secretion signal which is functional in said isolated host cells, b) a 6-methylguanine-DNA-methyltransferase enzyme (MGMT) or mutant thereof, wherein said MGMT enzyme or MGMT mutant is the protein of SEQ ID NO:2, or a homologous sequence thereof that is at least 80% identical to SEQ ID NO:2, and c) a recombinant protein. | ||
| 地址 | Paris FR | ||