PCR PRIMERS

摘要

The present disclosure provides methods, compositions, and kits for performing PCR (including multiplex PCR). The methods, compositions and kits provided herein use one or more primer pairs that contain one or more cleavable bases located at a minimal distance away from the 3′ termini of the primers, and increase the accuracy of downstream analysis of sequence data.

主权项

1. A method for performing polymerase chain reaction (PCR), comprising:
a) amplifying one or more different target nucleic acids in the presence of one or more different primer pairs specific to the one or more different target nucleic acids in a single reaction mixture via PCR, wherein each primer of the one or more different primer pairs contains one or more cleavable bases, and wherein in substantially all of the primers of the one or more different primer pairs, each of the one or more cleavable bases is at least 4, 5, 6, 7, or 8 nucleotides away from the 3′ terminus of the primer that comprises the one or more cleavable bases.