SAME-DAY BLOOD CULTURE WITH DIGITAL MICROSCOPY

摘要

Generally provided are methods for rapid culture of microorganisms in a sample, including methods for growth and recovery of live microbial cells directly from a sample. Various features include enabling growth of microorganisms in a sample along with a reduction of sample debris that may interfere with microorganism detection, and reduction in toxicities that may inhibit microorganism growth. Further methods for selectively degrading non-viable microbial cells, are provided, for enhanced detection of viable microbial cells following a growth period.

主权项

1. A method comprising:
obtaining a clinical specimen comprising a blood sample, wherein the blood sample comprises a microbial cell; diluting a portion of the clinical specimen with a culture medium to produce a diluted specimen; subjecting the diluted specimen to a growth period to produce a growth culture; and detecting the microbial cell from the growth culture.